Neomycin is an aminoglycoside antibiotic found in many topical medications such as creams, ointments, and eyedrops. The discovery of Neomycin dates back to 1949. It was discovered in the lab of Selman Waksman, who was later awarded the Nobel Prize in Physiology and medicine in 1951. Neomycin belongs to aminoglycoside class of antibiotics that contain two or more aminosugars connected by glycosidic bonds. Neamine (two rings), Ribostamycin (three rings), Paromomycin (four rings), and Lividomycin (five rings) are some other examples of aminoglycosides. They have shown tremendous potential as antibacterials. One of them, Gentamicin, has been used extensively in clinical practice. Due to the inherent oto- and nephrotoxicity of these substances, systemic use has declined, as safer alternatives have become available.